Figure 2. FGF-2 rapidly stimulates activation of Akt and ERK.
Hippocampal neurons were incubated for the specified periods of time with 100 ng/ml FGF-2, and lysed. Supernatant was analyzed by immunoblotting with antibody to A) phospho-Akt (p-Akt) and total Akt or B) phospho-ERK (p-ERK) and total ERK. Results are expressed as the means ± S.E.M. of the net intensities of bands obtained from 3 experiments, performed in duplicate. Experimental groups were compared by ANOVA followed by Dunnett’s multiple Comparison post tests. **, p<0.01 vs. control. Representative immunoblots are shown.