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. Author manuscript; available in PMC: 2008 Jun 18.
Published in final edited form as: Brain Res. 2007 Apr 19;1154:40–49. doi: 10.1016/j.brainres.2007.04.026

Figure 3. FGF-2 more effectively enhances survival than BDNF and IGF-1.

Figure 3

After the first 24 hours of routine culture, neurons were cultured for the subsequent 72 hours under low-insulin conditions in the presence of the specified concentrations of FGF-2 and/or 100 ng/ml IGF-1 or 20 ng/ml BDNF. Cell viability was assessed on day 4 of culture by MTT assays in which the yellow tetrazolium base compound is reduced by viable cells to a blue formazan product, detected at a wavelength of 570 nm (O.D.570). Results were normalized in each experiment by dividing the mean absorbance of treated cultures by the mean absorbance of nontreated cultures to obtain fold-control values, and are expressed as the means ± S.E.M. of 3 experiments, performed in duplicate. A) Experimental groups were compared by ANOVA followed by Dunnett’s multiple comparison post tests. ***, p<0.001 vs. control. B, C) Experimental groups were compared by ANOVA followed by Newman-Keuls Multiple Comparison post tests. *, 0.5; **, p<0.01; ***, p<0.001 vs. FGF-2 treatment.