Figure 3.

Rapid reaction of hTrxR with NADPH. Anaerobic reduction of 8 μM of enzyme with 5 eq of NADPH (after mixing). Times in milliseconds for each spectrum are as follows: 1, oxidized; 2, 9; 3, 31; 4, 63; and 5, 415. (Inset) Rapid reaction kinetic traces observed at 463 and 540 nm for the reduction of hTrxR by 5 eq of NADPH. Observed traces with fits to a sum of three exponentials. A_{540 nm} = 0.06–0.10 (0.01 for each marking) on the left axis and A_{463 nm} = 0–0.04 (0.01 for each marking) on the right y axis.