Figure 4. Influence of p70-specific antibodies on replication-related activities of RPA.

Antibodies were tested in SV40 DNA replication (A), primer synthesis (B) and primer extension (C) assays. In (A), each DNA was restricted with EcoRI alone (E, odd-numbered lanes) or double- restricted by EcoRI–DpnI (D, even-numbered lanes). The numbers below the lanes indicate the amounts (in pmol) of incorporated dNMPs (A, C) or rNMPs (B). Lane 1 contained no protein. The positions of the products of each reaction, linear double-stranded DNA (form II), RNA primers (RP) and RNA–DNA primers (RDP), are indicated. Markers contained oligo(dT)₈-₁₂ or an 81-base-pair fragment. Antibodies were present at the onset of the reaction (A) or before the unwinding (B) or primer synthesis (C) steps. The template configurations present at the time at which antibodies were administered were super-coiled, under-wound and primed plasmid DNAs and are represented by symbols for each reaction.