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. 2007 Sep 25;407(Pt 2):219–230. doi: 10.1042/BJ20070315

Figure 4. Acb1p-depleted cells exhibits an Opi− phenotype.

Figure 4

(A) Wild-type (Y700) and pGAL-ACB1 (Acb1p-depleted Y700) cells were tested for their ability to secrete inositol to the surrounding medium when supplemented with or without 0.5 mM palmitic acid. (B) Q-RT-PCR analysis of INO1 mRNA levels in wild-type (Y700, black bars) and pGAL-ACB1 (Acb1p-depleted Y700, white bars) cells grown in YNBD medium (without inositol) supplemented with or without 0.05% (v/v) Brij58 and 0.5 mM palmitic acid as indicated. (C) Immunoblot analysis of protein extracts from S. cerevisiae expressing Ino1p–HA. Wild-type (Y700 Ino1p–HA) and pGAL-ACB1 (Acb1p-depleted Y700 Ino1p–HA) cells were grown in YNBD medium (without inositol) supplemented with or without 0.05% (v/v) Brij 58 detergent and 0.5 mM palmitic acid as indicated. The blot was re-probed with an antibody against Vac8p to serve as a loading control. Data in (B) are means±S.D. (n=3) and the Western blot is representative of three independent experiments.