Table 2. Comparison of fold change (Acb1p depleted cells compared with wild-type cells) obtained by microarray and Q-RT-PCR analysis.
| Fold change* | |||||
|---|---|---|---|---|---|
| Gene ID | Gene | Function | Process | Microarray analysis | Q-RT-PCR† |
| Stress response | |||||
| ygr088w | CTT1 | Catalase T, cytosolic | Response to stress | No data‡ | 3.1±0.14 |
| ybr072w | HSP26 | Heat-shock protein | Response to stress | No data‡ | 5.9±1.4 |
| yol052c-a | DDR2 | Heat-shock protein DDRA2 | Response to stress | 6.9±0.71 | 2.7±0.66 |
| yll026w | HSP104 | Heat-shock protein | Response to stress | No data‡ | 3.1±0.41 |
| yfl014w | HSP12 | Heat-shock protein | Response to stress | No data‡ | 6.8±1.6 |
| C-compound and carbohydrate metabolism | |||||
| ydl022w | GPD1 | Glycerol-3-phosphate dehydrogenase (NAD+), cytoplasmic | Intracellular accumulation of glycerol | 6.4±1.8 | 5.5±0.89 |
| yjl052w | TDH1 | Glyceraldehyde-3-phosphate dehydrogenase 1 | Glycolysis; gluconeogenesis | 4.8±0.16 | 3.3±0.91 |
| Lipid metabolism | |||||
| ygl055w | OLE1 | Stearoyl-CoA desaturase | Fatty acid desaturation | 1.9±0.75 | 1.9±0.45 |
| yjl196c | ELO1 | Fatty acid elongation protein | Fatty acid elongation | No data‡ | 1.2±0.11 |
| ykl182w | FAS1 | Fatty-acyl-CoA synthase, β chain | Fatty acid biosynthesis | 1.6±0.25 | 1.6±0.41 |
| ypl231w | FAS2 | Fatty-acyl-CoA synthase, α chain | Fatty acid biosynthesis | 1.8±0.17 | 1.6±0.26 |
| yhr007c | ERG11 | Cytochrome P450 lanosterol 14α-demethylase | Ergosterol biosynthesis | No data‡ | -1.9±0.10 |
| yjl153c | INO1 | Myo-inositol-3-phosphate synthase | PI biosynthesis | No data‡ | 151±10.7 |
| ygr170w | PSD2 | Phosphatidylserine decarboxylase 2 | PS biosynthesis | No data‡ | 1.3±0.11 |
| ygr157w | CHO2 | Phosphatidylethanolamine N-methyltransferase | PC biosynthesis | 1.8±0.51 | 1.6±0.02 |
| yjr073c | OPI3 | Methylene-fatty-acyl-phospholipid synthase | PC biosynthesis | 2.9±0.68 | 6.5±0.55 |
| ynl169c | PSD1 | Phosphatidylserine decarboxylase 1 | PS biosynthesis | No data‡ | 1.3±0.17 |
| ypr113w | PIS1 | CDP-diacylglycerol–inositol 3-phosphatidyltransferase | PI biosynthesis | No data‡ | 1.1±0.07 |
| yer026c | CHO1 | CDP-diacylglycerol serine O-phosphatidyltransferase | PS biosynthesis | 1.8±0.24 | 1.7±0.14 |
| Others | |||||
| ypl154c | PEP4 | Aspartyl protease | Vacuolar protein, catabolism | 1.8±0.14 | 1.7±0.32 |
| yor061w | CKA2 | CK2 α′ chain | G2/M and G1/S transition etc. | No data‡ | −1.2±0.12 |
| yfl031w | HAC1 | Transcription factor | Unfolded protein response | No data‡ | 5.6±0.39 |
| ylr350w | ORM2 | Strong similarity to ORM1 | Response to unfolded protein | 2.8±0.43 | Not determined |
| ycl043c | PDI1 | Protein disulfide-isomerase precursor | Protein folding | 2.2±0.17 | Not determined |
| yjl034w | KAR2 | Nuclear fusion protein | Response to unfolded protein | 1.9±0.98 | Not determined |
*Positive values represent fold up-regulation in Acb1p-depleted cells compared with wild-type cells, whereas negative values represent fold down-regulation in Acb1p-depleted cells compared with wild-type cells. Data are means±S.D. Q-RT-PCR, n≥3; microarray analysis, n=2.
†For each Q-RT-PCR analysis, RNA from three or four different cell cultures were used for cDNA synthesis and mean values were calculated. ACT1 was used as endogenous reference.
‡Data are not given because value for intensities were below that of Arabidopsis controls or because data were not present in both (dye-reversal) arrays.