Table 3. The relative phospholipid content of Y700 pGAL-ACB1 cells compared with Y700 cells.
The content of a given phospholipid class in Acb1p-depleted cells (Y700 pGAL-ACB1) was normalized to wild-type (Y700) levels (wild-type=100%) and calculated using the following equation: Content=Σ {% individual phospholipid species in Wt/[(Wt 12C/13C)/(Δ 12C/13C)]}, where Δ is Y700 pGAL-ACB1 and wild-type (Wt) is Y700. Data are means±S.D. (n=2).
| Phospholipid content (%) | ||||||
|---|---|---|---|---|---|---|
| Medium | PA | PI | PG | PS | PE | PC |
| YPD | 95±7.8 | 128±15.2 | 89±18.9 | 78±2.5 | 129±7.8 | 98±15.5 |
| SC medium +0.2 mM inositol | 81±6.8 | 80±4.4 | 46±7.6 | 72±20.8 | 80±19.1 | 96±18.1 |
| SC medium without inositol | 120±22.5 | 223±0.9 | 66±8.4 | 125±18.9 | 139±23.5 | 100±15.9 |