Table 1. Morphometric analysis of the acidocalcisomes in T. brucei.

The number, circularity, diameter and absolute volume (calculated assuming acidocalcisomes are spheres) of the organelles were analysed and compared in control and TbVTC1 ablated cells. The cells submitted to RNAi (TbVTC1RNAi) were subdivided into three groups representing cells with fewer than ten, between 11 and 60, and more than 60 acidocalcisomes per cell. Results are expressed as means±S.D. *Significance as compared with wild-type cells (P<0.05).

	Number of acidocalcisomes	Mean circularity (nm)	Mean diameter (nm)	Absolute volume (×106 nm3)
Wild-type	36±11	0.95±0.02	194.6±19.26	4.83±1.69
TbVTC1 RNAi
≤10	8±2*	0.82±0.009*	674.2±194.4*	259.5±91.7*
11–60	28±7	0.84±0.07*	432.1±84.7*	82.3±60.5*
≥60	117±46*	0.89±0.01*	316.7±42.1*	25.7±12.3*