Figure 1. Time-dependence of the interaction between cyt c and phospholipid liposomes.

Horse cyt c (6 μM) and PC/PE/CL (5:4:3) liposomes (160 μM total phospholipid content) in 20 mM potassium phosphate (pH 7.0) were incubated at 37 °C. The liposomes with diameter ranging from 50 to 800 nm [50 nm (□), 100 nm (●), 400 nm (○) and 800 nm (■)] were prepared by extrusion through polycarbonate membranes with the respective pore sizes.