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Horse (■) or yeast (□) cyt c (6 μM) was titrated with PC/PE/CL (5:4:3) liposomes in 20 mM potassium phosphate buffer (pH 7.0) at 37 °C. [S]0.5 (concentration of CL required for half saturation) for horse cyt c binding was determined to be 10.0±1.9 μM, while that for yeast cyt c was found to be 22.1±1.8 μM.
