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. 2007 Aug 29;406(Pt 3):479–489. doi: 10.1042/BJ20070275

Figure 5. Sedimentation-velocity analysis of native and recombinant human SP-A.

Figure 5

Data were obtained with 0.2 mg/ml SP-A subjected to centrifugation at 35000 rev./min in buffer A, at 25 °C. Concentration distribution versus sedimentation coefficient shows different oligomerization states for recombinant SP-As, which would be compatible with trimers and hexamers (for SP-A1), and higher supratrimeric assemblies (for SP-A2 and SP-A1/SP-A2). Native human SP-A is highly oligomerized and multimerized in solution. The protein exhibited two major species peaks with sedimentation coefficients of 12 and 17.5 S. Results are consistent with the presence of heterogeneity. The results shown are from a representative one of two or three experiments.