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. 2007 Aug 29;406(Pt 3):437–444. doi: 10.1042/BJ20070390

Figure 3. p53 binds to the PC4 promoter in vivo.

Figure 3

HEK-293 cells were treated with doxorubicin for 24 h and ChIP assays were performed using p53 antibody. (A) Sequences of the p53-binding sites in the PC4 promoter. p53.1 F and R, p53.2 F and R, p53.3 F and R, and p53.4 F and R represent the primer sets used for PCR amplification of the respective sites. The amplicon sizes and primer sets are labelled. (B) ChIP assay was performed with or without anti-p53 antibody. The final DNA extractions were amplified using specific primers for p53-binding sites in PC4 (I) and p21 (II) promoters. (C) The fold enrichment of p53 recruitment upon doxorubicin treatment in p53-binding sites of the PC4 promoter and p21 promoter.