Figure 5. p53-dependent overexpression of PC4.

(A, B) H1299 (p53^−/−) cells were treated with cisplatin for 12 h and doxorubicin for 24 h. RNA was isolated and real-time PCR analysis was performed using specific primers for PC4 and p21 with β-actin as a control. (C) H1299 (p53^−/−) cells were treated with doxorubicin for 24 h. Whole cell lysates were made from the treated/untreated cells and immunoblotting was performed for PC4, p53 and β-actin.