Table 1. Oligonucleotides used in EMSA and p53 binding constants.
Primer | Sequence | Kd |
---|---|---|
p53.1 | GTCATTTCTGCTTTGGAACATGCTTGTTAGGATGAGTAAT | 4.5 nM |
p53.2 | GATTTTAGGAGGCAGAGGCATGTATTGAGGTGGGTGAAGT | 9.3 nM |
p53.3 | TGAACAAACGCAAAGCTTGGCGCTTGTCACTCATGTTTTAT | 17.8 nM |
p53.4 | TGGTTTCTGTGGGGAGGCCACGCCCCATCACGTGACCGCA | 22.0 nM |
GADD | AATTCTCGAGCAGAACATGTCTAAGCATGCTGGGCTCGAG | 4.1 nM |