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. 2007 Aug 29;406(Pt 3):437–444. doi: 10.1042/BJ20070390

Table 1. Oligonucleotides used in EMSA and p53 binding constants.

Sequence and binding constant values (Kd) of each p53-binding site in the PC4 promoter, along with the GADD45 p53-binding site are shown.

Primer Sequence Kd
p53.1 GTCATTTCTGCTTTGGAACATGCTTGTTAGGATGAGTAAT 4.5 nM
p53.2 GATTTTAGGAGGCAGAGGCATGTATTGAGGTGGGTGAAGT 9.3 nM
p53.3 TGAACAAACGCAAAGCTTGGCGCTTGTCACTCATGTTTTAT 17.8 nM
p53.4 TGGTTTCTGTGGGGAGGCCACGCCCCATCACGTGACCGCA 22.0 nM
GADD AATTCTCGAGCAGAACATGTCTAAGCATGCTGGGCTCGAG 4.1 nM