Table 1. Phenotypic analysis of the F1 generation of acn2 and cts-1 crosses.
The phenotypes tested were the proportion of total seeds germinated by 4 days after transfer to the growth chamber and establishment in the presence of FAc. FAcR, fluoroacetate resistance; NA, not applicable; suc, sucrose.
| Genotype | MS* (%) | MS+Suc† (%) | FAcR‡ (%) |
|---|---|---|---|
| Col-0 (n=3) | 99±1 | NA | 0 |
| Ler (n=3) | 100 | NA | 0 |
| cts-1 (n=3) | 11±4 | 53±2 | >99 |
| acn2 (n=3) | <1 | 35±4 | >99 |
| cts-1×acn2 (n=6) | 5±2 | 49±17 | >99 |
| Ler×acn2 (n=3) | 100 | NA | 0 |
| Ler×cts-1 (n=3) | 100 | NA | 0 |
| Col-0×acn2 (n=3) | 96±2 | NA | 0 |
*Approx. 100 seeds were sown on to agar plates containing half-strength MS salts, pH 5.7. The plates were incubated in the dark for 4 days before being transferred to the growth cabinet.
†Seeds that had not germinated were tranferred on to agar plates containing half-strength MS salts and 20 mM sucrose.
‡Seeds were sown directly on to agar plates containing half-strength MS salts, pH 5.7, 20 mM sucrose and 0.5 mM FAc. Seeds from mutants and mutant crosses were nicked 24 h after transfer to the growth cabinet.