Figure 1. Silver-stained gel after 2D IEF–SDS/PAGE separation of proteins from Arabidopsis thylakoid associated polysomes.
The polysomes were isolated from WT Arabidopsis plants grown under standard conditions; proteins were separated by 2D electrophoresis with denaturing IEF on immobilized pH gradient between 4 and 7 in the first dimension and with SDS/PAGE in the second dimension. The protein spots were identified with MALDI–TOF (identified proteins are listed in Table 1).