(A) Thylakoid membranes isolated from WT plants were subjected to 2D BN-PAGE; proteins were transferred to a PVDF membrane, followed by the analysis with antibodies against D1, CP43 and TLP18.3. (B) Thylakoid membranes were fractionated into stroma (ST) and grana (GT) thylakoids by digitonin, and proteins were separated by SDS/PAGE, followed by transfer to PVDF membrane and analysis with antibodies against TLP18.3, CP43, Lhcb4 and PsaA/B.