Table 3. Comparison of the rate constants (kPI) for D1 protein degradation and the relative synthesis rates of the D1 protein in the WT and ΔTLP18.3 thylakoids.
Detached leaves from WT and ΔTLP18.3 plants were pulse labelled under HL (900 μmol of photons·m−2·s−1) with [35S]methionine for 15, 30 and 60 min, followed by 1, 2 and 3 h chase in the presence of unlabelled methionine (see Supplementary Figure 2 at http://www.BiochemJ.org/bj/406/bj4060415add.htm). (A) kPI and half-times (T½, min) for the D1 protein degradation were calculated from data fitted to first-order reaction kinetics (r=k[A]). Data shown are means±S.D., n=3 for WT and GABI-Kat 459D12 mutant and n=1 for SALK_109618 mutant. (B). Relative D1 protein synthesis rates were determined by comparing the amount of radioactivity incorporated into the D1 protein normalized to that incorporated into ATP synthase α-/β- subunits in WT and ΔTLP18.3 thylakoids during radioactive pulse of 15 min at HL. Data shown are means±S.D., n=3.
| (A) | ||
|---|---|---|
| Lines | kPI×103, min−1 | T½, min |
| WT | 8.1±1.1 | 87±13 |
| GABI-Kat 459D12 | 4.4±1.1 | 163±42 |
| SALK_109618 | 5.0 | 138 |
| (B) | ||
| Lines | Relative synthesis rate | |
| WT | 1.00±0.00 | |
| GABI-Kat 459D12 | 0.69±0.15 | |
| SALK_109618 | 0.69±0.05 |