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Schematic of primer locations for the generation of artificial template for IVS 2-2 A>G mutation. A: IVS 2-2 forward primer; B: IVS 2-2 reverse primer; C: artificial oligodeoxyribonucleotides template forward primer; and D: artificial oligodeoxyribonucleotides template reverse primer. “X” indicates the position of the mutation.
