Table 1.

Oligodeoxyribonucleotide Design for Artificial Template IVS2-2A>G Mutation

Artificial template forward-IVS2-2F (90 bp)

5′ -CAGGTAACTGGTGGTATGGGGCAGTGAGTGCTTCTAGCCTATCCTTGTCGGTGGGTGAATCCCCAGTACGATAGCACCTTCCTGTTTGAC-3′

Artificial template reverse-IVS2-2R (91 bp)

5′ -AGCTAGTTGGAGCCAGGTTACCTGGACTGGGGGCATCAGGCTGCAGAGCTGGGAAGTCGTTGTCAAACAGGAAGGTGCTATCGTACTGGGG-3′

The mutation is shown in underline bold italic; the overlapping region is in bold; and short primers are underlined.