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Methylation analysis of different fragments of DAPK promoter. CaSki cell DNA was methylated in vitro by SssI methylase and compared with the same untreated DNA preparation. Ms-DMSO-PCR: 5 to 10 ng of each DNA template were taken for each PCR reaction with the concentration of DMSO ranging from 1 to 5%. L, 100-bp DNA ladder. Primers and their location in promoter region are shown in Figure 3 and Table 2.
