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. 2007 Nov;9(5):574–581. doi: 10.2353/jmoldx.2007.070025

Figure 6.

Figure 6

Sensitivity of Ms-DMSO-PCR to time course methylation and detection of methylation in CaSki and SiHa DNA mixtures. A: Ms-DMSO-PCR of DAPK promoter of DNA from CaSki cells methylated in vitro for 0, 30, 60, and 120 minutes: 10 ng of each DNA template were taken for each PCR reaction with the concentration of DMSO ranging from 1 to 5%. B: PCR of MspI- and HpaII-digested DNA of the same samples. N, Noncut DNA; M, MspI digest; H, HpaII digest. C: Ms-DMSO-PCR of DAPK promoter from CaSki and SiHa DNA mixtures: 10 ng of each DNA template or mixture were taken for each PCR reaction with the concentration of DMSO ranging from 1 to 4%.