Figure 7.
Ms-DMSO-PCR of DAPK and MGMT promoters in clinical samples of the uterine cervix: 10 ng of each DNA template were taken for each PCR reaction with the concentration of DMSO ranging from 2 to 5% for frozen biopsies and 0 to 2% for FFPE tissue. Nl cells, HPV−, normal cells, HPV-negative; SCC HPV+, squamous cell carcinoma, HPV-positive. A: Demethylation of DAPK promoter in frozen biopsies of SCC. B: Methylation of MGMT promoter in frozen biopsies of SCC. C: Demethylation of DAPK promoter in FFPE tissues of SCC.