Table 1.
Primer Sequences
| Gene name | GenBank no. | Primers and annealing temperature (Tann) | Location* | Size† (bp) |
|---|---|---|---|---|
| Death-associated protein kinase 1 | NT_023935.17 | F:5′-CAGTACACATCTCGGGACGG-3′ | −371+24 | 395 |
| (DAPK) | R:5′-GTCCCCGGCGTTGGCTCG-3′ | |||
| Tann = 58°C | ||||
| O-6-methylguanine-DNA | AL355531 | F:5′-GGTCGCTTGCACGCCCGC-3′ | −435−102 | 333 |
| methyltransferase (MGMT) | R:5′-AAGAGAGCGCGCGGGGGC-3′ | |||
| Tann = 70°C | ||||
| Ras association domain family 1, | NM_007182 | F:5′-CGCTGAAGTCGGGGCCCG-3′ | +45+371 | 326 |
| transcript variant A (RASSF1A) | R:5′-CAGGCCTTTGCGCACGACG-3′ | |||
| Tann = 62°C | ||||
| Tissue inhibitor of metalloproteinase 3 | NT_011520.10 | F:5′-CCCCAACAGCCCGAGGCG-3′ | +825+1192‡ | 367 |
| (TIMP3) | R:5′-TTGCCGCTGCTGCCGCCG-3′ | |||
| Tann = 63°C |
*
Location of CpG island to the start of transcription.
†
Size of PCR product.
‡
Start of translation is at 1193 bp from start of transcription.