Table 2.
Size of PCR product (bp) | Location of PCR product to the start of transcription | Primer name and annealing temperature (Tann) | Number of CG in primer | Number of CG in PCR product | C + G content in PCR product (%) |
---|---|---|---|---|---|
161 | −137 | Up 3 | 1 | 16 | 74.5 |
+24 | Down 2 | 3 | |||
Tann = 60°C | |||||
395 | −371 | Up 2 | 2 | 45 | 72.4 |
+24 | Down 2 | 3 | |||
Tann = 58°C | |||||
448 | −424 | Up 1 | 0 | 47 | 70.3 |
+24 | Down 2 | 3 | |||
Tann = 58°C | |||||
649 | −137 | Up 3 | 1 | 65 | 73.2 |
+512 | Down 1 | 0 | |||
Tann = 64°C | |||||
883 | −371 | Up 2 | 2 | 95 | 72.6 |
+512 | Down 1 | 0 | |||
Tann = 60°C | |||||
936 | −424 | Up 1 | 0 | 97 | 71.6 |
+512 | Down 1 | 0 | |||
Tann = 60°C |