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. 2007 Nov;9(5):574–581. doi: 10.2353/jmoldx.2007.070025

Table 2.

Primers and Characterization of PCR Products of DAPK Promoter

Size of PCR product (bp) Location of PCR product to the start of transcription Primer name and annealing temperature (Tann) Number of CG in primer Number of CG in PCR product C + G content in PCR product (%)
161 −137 Up 3 1 16 74.5
+24 Down 2 3
Tann = 60°C
395 −371 Up 2 2 45 72.4
+24 Down 2 3
Tann = 58°C
448 −424 Up 1 0 47 70.3
+24 Down 2 3
Tann = 58°C
649 −137 Up 3 1 65 73.2
+512 Down 1 0
Tann = 64°C
883 −371 Up 2 2 95 72.6
+512 Down 1 0
Tann = 60°C
936 −424 Up 1 0 97 71.6
+512 Down 1 0
Tann = 60°C