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. Author manuscript; available in PMC: 2007 Nov 2.
Published in final edited form as: FASEB J. 2006 May 23;20(9):1498–1500. doi: 10.1096/fj.05-5319fje

Figure 3.

Figure 3

Effect of LPS stimulation on ANXA1 phosphorylation. Membrane attached (A) and cytosolic fraction (B) of ANXA1 was analyzed for its phosphorylation on serine 27. Western blot analysis expressed as % of unstimulated cells (C) as optical density arbitrary units. C) Equal amount of EDTA detached ANXA1 from LPS-stimulated cells were treated with 10 U alkaline phosphatase for 1 h at 37°C. Then, Western blot with 2 different antibodies: one of which discriminates between the phosphorylated or not was performed. One representative of at least 3 independent experiments is shown.