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. Author manuscript; available in PMC: 2007 Nov 2.
Published in final edited form as: FASEB J. 2006 May 23;20(9):1498–1500. doi: 10.1096/fj.05-5319fje

Figure 4.

Figure 4

Effects of PI3 kinase and MAPK inhibitors on ANXA1-S27 phosphorylation. Cells were pretreated for 30 min with LY 294002 (LY, 1.4 mM) or PD 98059 (PD, 5 mM) or with a neutralizing Ab against IL-6 (50 ng/ml) and then LPS was added for further 5 min. ANXA1-S27-PO4 was measured by FACS; % of positive cells to immunostaining is reported. A control Ab (matched isotype) was used as an internal control. *P < 0.025 vs. unstimulated, **P < 0.01 vs. LPS stimulated (ANOVA and Bonferroni test).