Figure 4.

Reduced CD3⁺ and CD11c⁺ cell interaction in lung tissue of sensitized and challenged Arhgef1^−/− mice. (A) Histologic lung sections stained with anti-CD3 (blue) and anti-CD11c (red). Green color is autofluorescence and is used to identify lung tissue. Top panels are ×10 original magnification and bottom panels ×40 original magnification. Arrows indicate CD3⁺/CD11c⁺ contacts. (B) Number of CD3⁺ T cells, CD11c⁺ cells, and CD3⁺-CD11c⁺ cell contacts per 10× field of view in sensitized and challenged wild-type (WT) and Arhgef1^−/− mice. Average numbers were obtained from at least four fields of view per section from four different sections per set of lungs and from four individual mice. *p < 0.05 by Student's t test.