Figure 5.

Arhgef1-deficient antigen-specific Th2 cytokine production by isolated leukocytes but not explant organ cultures. (A) Amount of IL-13 produced in response to media, ovalbumin (OVA) (100 μg/ml), or anti-CD3 + anti-CD28 (both 1 μg/ml) (CD3/CD28) from cultures of isolated leukocytes from sensitized and challenged wild-type (WT) and Arhgef1^−/− spleens (left panel) and lungs (right panel). Symbols represent the average response from individual experiments, and the bar is the mean of experiments. *p ⩽ 0.05 by Student's paired, two-tailed t test. (B) Proliferation of WT and Arhgef1^−/− lung and spleen leukocytes in response to media or OVA (100 μg/ml). Data from lungs (right side) are representative of two experiments, and data from spleen (left side) represent three experiments. (C) IL-13 production from spleen (left panel) and lung (right panel) explant cultures treated with media, OVA, or anti-CD3/CD28 at same concentrations as described previously here. Values are expressed as pg of cytokine/mg of dry tissue and represent the mean ± SEM of three experiments. *p ⩽ 0.05 by Student's paired, two-tailed t test.