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. 2007 Oct 29;408(Pt 1):123–130. doi: 10.1042/BJ20070687

Figure 7. Effects of lactate on intact mice skeletal muscle PFK activity and on the F1,6BP/F6P ratio in C2C12 myoblasts.

Figure 7

(A) Muscle slices were incubated for 2 h in Krebs–Ringer–Henseleit buffer as described in the Experimental section, in the absence and presence of 100 nM insulin. After incubation, muscles were homogenized in the same buffer and PFK activity was measured. The absolute PFK activity in the absence of lactate and insulin was 5.9±0.4 m-units/mg of protein. *P<0.05 compared with the control in the same group. (B) Confluent C2C12 cells were incubated for 2 h in the absence and the presence of 10 mM lactate. After incubation cells were harvested, disrupted and the F1,6BP and F6P content were measured. Values are means±S.E.M. for three independent experiments. *P<0.05 compared with the control.