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. 2007 Oct 29;408(Pt 1):113–121. doi: 10.1042/BJ20070786

Figure 3. Methylation on H4-R3 occurs in vivo.

Figure 3

Recombinant H4 (upper panel, lane 1) and equivalent amount of calf thymus histones that were either subjected to mock (upper panel, lane 2) or AtPRMT1a methylation (upper panel, lane 3), together with total histones purified from A. thaliana (At) (upper panel, lane 4), were separated by SDS/15% PAGE gel and followed by Western blot analysis using the anti-H4R3ame2 antibody (upper panel). Then the membrane was re-probed with anti-H4 antibody for roughly equal loading (lower panel). All of the relevant proteins are indicated at the top.