Figure 1. Effects of p16INK4a and E2F binding on expression of ZFHX1A/B and Snail1/2 family members.

U2OS cells stably expressing IPTG-inducible p16INK4a and OHT-dependent mER–DB-E2F (where DB is a DNA-binding domain) were treated with IPTG, OHT or IPTG + OHT as described in the Experimental section. mRNA expression was followed by real-time PCR and compared with untreated cells (Ctrl). Results were normalized to β-actin mRNA. Similar results were seen with normalization to GAPDH mRNA. RRM2, ribonucleotide reductase.