Figure 6. E2Fs bind the ZFHX1A promoter in vivo, and Rb and HDAC2 are recruited to the promoter in wild-type MEFs but not TKOs.

(A) Real-time PCR was used to quantify ChIP assays at the ZFHX1A promoter. Antibodies used for immunoprecipitation are shown at the bottom. (B and C) Gels following real-time PCR amplification are shown for controls: the GAPDH promoter and an internal sequence within the ZFHX1A gene. ‘Pan’ indicates antibodies recognizing all forms of histone H3 and H4, and ‘ace’ indicates antibodies specific for acetylated histones. IgG, control antibody.