Figure 2. Acute absorption of radiolabelled lipids in the liver and serum of wild-type and NPC⁻ mutant mice.

A dose containing [4-¹⁴C]cholesterol (crossed-hatched bars) and [9,10-³H (n)]trioleoylglycerol (‘Triolein’, hatched bars) was orally gavaged into wild-type (WT), NPC1-deficient (NPC1) and NPC2-deficient (NPC2) mice. (A) After 2 h, lipids were measured in the intestine, liver and serum (results are means±S.E.M.). (B) Absorbed lipids were calculated as the percentage absorption out of the intestine (liver+serum) relative to total uptake (intestine+liver+serum). Error bars represent S.E.M. for each genotype set, which typically included four or five animals. Analysis of variance did not reveal significant differences among the genotypes (P>0.1).