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. 2007 Oct 29;408(Pt 1):105–111. doi: 10.1042/BJ20070817

Figure 5. TLE1 is unable to repress NF-κB-dependent gene expression in MEFs that are null for Sirt1.

Figure 5

(A) MEFs that are homozygous Sirt1-null (−/−) or heterozygous SIRT1-null (−/+) were transfected with 50 ng 5×-κB-TATA-Luc reporter plasmid and 500 ng of the indicated expression plasmids. pCMV-3B was used as the vector control. Renilla luciferase was used as the internal control for transfection. At 24 h post-transfection, cells were induced with TNFα for 3 h and harvested for luciferase assay. (B–D) In the presence of exogenously expressed Sirt1, TLE1 is able to repress NF-κB-dependent gene expression in Sirt1-null MEFs. MEFs that are wild-type (B), heterozygous Sirt1-null (C) or homozygous Sirt1-null (D) were transfected with 50 ng of 5×-κB-TATA-Luc reporter plasmid and 500 ng of the indicated expression plasmids. Renilla luciferase was used as the internal control for transfection. At 24 h post-transfection, cells were induced with TNFα for 3 h and harvested for luciferase assay. The results are presented as percentage luciferase activities.