Table 3. Transamination half-reaction kinetic parameters.
*No saturation detected at [L-phenylalanine] and [L-aspartate] up to 100 mM. †Data can be fitted equally well to eqn (9) without the term kr.
| kmax (s−1) | Kappm(mM) | kmax/Kappm (mM−1·s−1) | kr (s−1) | |
|---|---|---|---|---|
| L-Alanine | 49±5 | 38±9 | 1.3±0.3 | 6±2 |
| L-Serine | 15±3 | 213±76 | 0.070±0.03 | 0.3±0.1† |
| L-Phenylalanine* | – | – | 0.005±0.001 | 0.1±0.05† |
| L-Arginine | 0.134±0.005 | 51±6 | 2.6×10−3±3×10−4 | – |
| L-Glutamate | 0.032±0.003 | 409±91 | 8×10−5±2×10−5 | – |
| L-Aspartate* | – | – | 1×10−9±1×10−10 | – |
| Glyoxylate | 49±1 | 0.22±0.01 | 220±11 | 0.4±0.3† |
| Pyruvate | 15±1 | 0.3±0.06 | 50±10 | – |