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. Author manuscript; available in PMC: 2008 Feb 1.
Published in final edited form as: Free Radic Biol Med. 2006 Nov 10;42(3):385–393. doi: 10.1016/j.freeradbiomed.2006.11.009

Fig 3.

Fig 3

Smoke inhalation modulates BER in the hippocampus. In vitro cleavage of oxidative adducts by hippocampal nuclear extracts from control rats and rats at 2, 6, 24 hours and 7 days post smoke is shown: (A) Autoradiograms of incision products (P) generated over time by cleavage of end labeled double stranded oligonucleotide substrates (S) carrying either the *8-oxodG/C adduct or the abasic site *AP[THF]/A. [−] is a negative control in absence of extract; OGG1 and APE1 lanes show reference reactions assembled with the respective recombinant enzymes. (B) Values from Phosphorimager quantitation of products generated with extracts from 4 rats per group and 3 cleavage assays per extract were averaged and plotted as mean ± SD. * indicates cleavage significantly different from control for each respective time point P<0.05.