Skip to main content
. 2007 Nov 15;21(22):2950–2962. doi: 10.1101/gad.1592807

Figure 1.

Figure 1.

Association of RNAP II with active genes during the cell cycle. (A) Extracts were prepared from cells released from G1/S-phase-arrested HeLa cells. ChIP experiments were performed with anti-RNAP II H14 antibody, and products were amplified with primers for the β-actin gene at the promoter, coding, and poly(A) regions and with primers for a 28S rRNA gene. Quantitative data were obtained by subtracting the values of each PCR product from the signal of mock antibody with anti-mouse IgM. Standard errors were calculated from at least three independent experiments. (B) ChIP experiments were performed as in A but with anti-RNAP II H5 antibody, and the products were amplified with primers for the β-actin gene at the promoter, coding, and poly(A) regions and with primers for a 28S rRNA gene.