Figure 4.

Evidence that TRX associated with Ref-1 through the redox-active cysteines in vitro. Various TRX mutants containing cysteine-to-serine or cysteine-to-alanine substitutions were cross-linked with Ref-1 or wbFos by diamide. After incubation, the complexes were resolved by electrophoresis under reducing or oxidizing conditions and detected by antibodies. (A) Western blot analysis of various TRX and Ref-1 complex by use of anti-Ref-1 antibody. (B) Western blot analysis of TRX^{C32S/C69S/C73S} and Ref-1 complex by use of anti-TRX antibody. (C) Western blot analysis of TRX^{C32S/C69S/C73} and wbFos by use of anti-c-Fos antibody or anti-TRX antibody.