Figure 5.

Demonstration of a physical association between TRX and Ref-1 by mammalian two-hybrid assay in COS-7 cells. (A) An association between wild-type TRX and Ref-1 was monitored by a luciferase assay. (B) An association between TRX mutants and Ref-1 was monitored by a luciferase assay. The relative fold induction of luciferase activity was calculated by normalizing to the β-galactosidase activity. The results are the means ± SE of three experiments (each in duplicate) and presented as fold increases in luciferase activity over the baseline seen in lane 1.