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In vitro characterization of all-R-K13 ligand. (A) Competition for 125I-PTH-(1–34)-binding in HEK-293/C-21 cells by all-R-K13 (line with open figures) and [Nle8,18,Tyr34]bPTH-(1–34)NH2 (line with filled figures). (B) Dose–response curves for the stimulation of adenylyl cyclase activity in Saos-2/B-10 cells by all-R-K13 (line with open figures) and [Nle8,18,Tyr34]bPTH-(1–34)NH2 (line with filled figures). Similar results were obtained in two additional experiments.
