Abstract
The gene coding for an antigenic 34-kDa protein of Mycobacterium paratuberculosis was isolated and sequenced. The 897-bp open reading frame coded for a novel protein containing specific B epitopes. The occurrence of well-defined hydrophobic and hydrophilic portions suggests the wall location of the protein.
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- Aldovini A., Debouck C., Feinberg M. B., Rosenberg M., Arya S. K., Wong-Staal F. Synthesis of the complete trans-activation gene product of human T-lymphotropic virus type III in Escherichia coli: demonstration of immunogenicity in vivo and expression in vitro. Proc Natl Acad Sci U S A. 1986 Sep;83(18):6672–6676. doi: 10.1073/pnas.83.18.6672. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Barnes P. F., Mehra V., Hirschfield G. R., Fong S. J., Abou-Zeid C., Rook G. A., Hunter S. W., Brennan P. J., Modlin R. L. Characterization of T cell antigens associated with the cell wall protein-peptidoglycan complex of Mycobacterium tuberculosis. J Immunol. 1989 Oct 15;143(8):2656–2662. [PubMed] [Google Scholar]
- Cherayil B. J., Young R. A. A 28-kDa protein from Mycobacterium leprae is a target of the human antibody response in lepromatous leprosy. J Immunol. 1988 Dec 15;141(12):4370–4375. [PubMed] [Google Scholar]
- De Kesel M., Gilot P., Coene M., Cocito C. Composition and immunological properties of the protein fraction of A36, a major antigen complex of Mycobacterium paratuberculosis. Scand J Immunol. 1992 Aug;36(2):201–212. doi: 10.1111/j.1365-3083.1992.tb03092.x. [DOI] [PubMed] [Google Scholar]
- De Kesel M., Gilot P., Misonne M. C., Coene M., Cocito C. Cloning and expression of portions of the 34-kilodalton-protein gene of Mycobacterium paratuberculosis: its application to serological analysis of Johne's disease. J Clin Microbiol. 1993 Apr;31(4):947–954. doi: 10.1128/jcm.31.4.947-954.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Edman P., Begg G. A protein sequenator. Eur J Biochem. 1967 Mar;1(1):80–91. doi: 10.1007/978-3-662-25813-2_14. [DOI] [PubMed] [Google Scholar]
- Gilot P., De Kesel M., Coene M., Cocito C. Induction of cellular immune reactions by A36, an antigen complex of Mycobacterium paratuberculosis: comparison of A36 and johnin components. Scand J Immunol. 1992 Dec;36(6):811–821. doi: 10.1111/j.1365-3083.1992.tb03143.x. [DOI] [PubMed] [Google Scholar]
- Hawley D. K., McClure W. R. Compilation and analysis of Escherichia coli promoter DNA sequences. Nucleic Acids Res. 1983 Apr 25;11(8):2237–2255. doi: 10.1093/nar/11.8.2237. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hirschfield G. R., McNeil M., Brennan P. J. Peptidoglycan-associated polypeptides of Mycobacterium tuberculosis. J Bacteriol. 1990 Feb;172(2):1005–1013. doi: 10.1128/jb.172.2.1005-1013.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kyte J., Doolittle R. F. A simple method for displaying the hydropathic character of a protein. J Mol Biol. 1982 May 5;157(1):105–132. doi: 10.1016/0022-2836(82)90515-0. [DOI] [PubMed] [Google Scholar]
- Marck C. 'DNA Strider': a 'C' program for the fast analysis of DNA and protein sequences on the Apple Macintosh family of computers. Nucleic Acids Res. 1988 Mar 11;16(5):1829–1836. doi: 10.1093/nar/16.5.1829. [DOI] [PMC free article] [PubMed] [Google Scholar]
- McNeil M. R., Brennan P. J. Structure, function and biogenesis of the cell envelope of mycobacteria in relation to bacterial physiology, pathogenesis and drug resistance; some thoughts and possibilities arising from recent structural information. Res Microbiol. 1991 May;142(4):451–463. doi: 10.1016/0923-2508(91)90120-y. [DOI] [PubMed] [Google Scholar]
- Porath J., Carlsson J., Olsson I., Belfrage G. Metal chelate affinity chromatography, a new approach to protein fractionation. Nature. 1975 Dec 18;258(5536):598–599. doi: 10.1038/258598a0. [DOI] [PubMed] [Google Scholar]
- Shine J., Dalgarno L. Determinant of cistron specificity in bacterial ribosomes. Nature. 1975 Mar 6;254(5495):34–38. doi: 10.1038/254034a0. [DOI] [PubMed] [Google Scholar]
- Short J. M., Fernandez J. M., Sorge J. A., Huse W. D. Lambda ZAP: a bacteriophage lambda expression vector with in vivo excision properties. Nucleic Acids Res. 1988 Aug 11;16(15):7583–7600. doi: 10.1093/nar/16.15.7583. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Thole J. E., Stabel L. F., Suykerbuyk M. E., De Wit M. Y., Klatser P. R., Kolk A. H., Hartskeerl R. A. A major immunogenic 36,000-molecular-weight antigen from Mycobacterium leprae contains an immunoreactive region of proline-rich repeats. Infect Immun. 1990 Jan;58(1):80–87. doi: 10.1128/iai.58.1.80-87.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]