Figure 1.
Analysis of the interaction of Tax with several hTAFIIs. (A–C) COS-7 cells were transfected with pSG-Tax (14) either alone or together with a vector expressing the hTAFII (12) indicated above the lanes. Amounts of transfected plasmids were as follows: 2 μg, pSGTax, pXJ41-hTAFII30, or pXJ41-hTAFII55; 5 μg, pXJ41-hTAFII18, pXJ41-hTAFII28, pXJ41-hTAFII100, or pXJ41-hTAFII135; 10 μg, pXJ41-hTAFII20 or pXJ41-hTAFII250. Under these conditions equal amounts of the various hTAFII proteins were produced (data not shown). Cell extracts were precipitated with mAbs specific of the corresponding hTAFII (16TA for hTAFII18, 22TA for hTAFII20, 15TA for hTAFII28, 2F4 for hTAFII30, 19TA for hTAFII55, 1TA for hTAFII100, 20TA for hTAFII135) (10, 13, 15). The precipitated proteins were revealed by immunoblotting using a rabbit polyclonal antibody directed against Tax (16). The coprecipitated Tax protein is shown by an arrow.