Figure 4.

Overexpression of hTAF_II28 and TBP alleviate GAL4-Tax self-squelching. The reporter construct pG3G4CAT (1.5 μg) was transfected in HeLa cells with increasing amounts of pSG4-Tax (0, 10, 20, 40, and 100 ng). Quantification of CAT protein was done as described in the legend to Fig. 3. CAT concentrations in picograms per milliliter of cellular extract were plotted against the amount of transfected pSG4Tax (−). The same experiment was done with inclusion of the following expression vectors in the DNA mix: pXJ41-hTAF_II28 (+hTAF_II28), pSG-TBP (+TBP), and pXJ41-hTAF_II28 with pSG-TBP (+TBP+hTAF_II28). The amount of pXJ41-hTAF_II28 and pSG-TBP used in the transfection was 1 μg.