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. 1997 Apr 15;94(8):3662–3667. doi: 10.1073/pnas.94.8.3662

Figure 5.

Figure 5

The effect of the overexpression of hTAFII28 is lost with mutants of Tax unable to bind hTAFII28. (A) COS-7 cells were cotransfected with the following Tax expression vectors (7, 14, 15): pSG-Tax (1 μg; lane 2), pcTax M5 (5 μg; lane 3), pcTax M15 (5 μg; lane 4) and pSG-Tax M137 (2 μg; lane 5). pXJ41-hTAFII28 (2 μg) was included in the DNA mix for lanes 2–5. Cell extracts were precipitated with mAb 15TA directed against hTAFII28. The precipitated proteins were analyzed by immunoblotting using a rabbit polyclonal antibody directed against Tax (17). The coprecipitated Tax protein is shown by an arrow. (B) HeLa cells were cotransfected with pSG4-Tax, pSG4-Tax M5, pSG4-Tax M15, and pSG4-Tax M137 with or without pSG-TBP and pXJ41-hTAFII28 as indicated. Twenty nanograms of plasmids expressing the Tax fusion proteins was used. For pSG-TBP and pXJ41-hTAFII28, 1 μg of plasmid was used. Quantification of CAT enzyme was done as described in the legend to Fig. 3.