Figure 1.
Differential localization of Bax and Bcl-XL and their association with membranes in response to dexamethasone-induced apoptosis. Apoptosis was induced in murine thymocytes by the addition of dexamethasone. Untreated cells (lanes a–c) and cells incubated 2 h (lanes d–f) and 4 h (lanes g–i) with dexamethasone were hypotonically lyzed, homogenized with a Dounce homogenizer, and separated into soluble (lanes a, d, and g), high-speed membrane pellet (lanes b, e, and h), and nuclear fractions (lanes c, f, and i) by differential centrifugation. The protein samples were analyzed by SDS/PAGE on gels stained with Coomassie blue (CB) or by Western blotting with anti-mBax 5B7, anti-mBcl-2 10C4, and anti-uBcl-XL 2H12 monoclonal antibodies.