Table 2.

Sequence alignments of LRRs

LRR	Species	Sequence	C-terminal residue
1	A.c.	HVKKFNMKEFEQPVTPNELKA-------LVDTLHFNN	224
	C.e.	KLRDLKIDDFSH-LLPKDLLP-------IVGVLQYSA	221
2	A.c.	YFIGF-VARDYRLDKDSMK--------VLTDMIGVNT	253
	C.e.	YFTGL-ICDAVRVSSEVID--------VVLSVIRKSQ	250
3	A.c.	KMEELVLSRVEGTASAFE---------ELGQAIAKNRNS	283
	C.e.	NLKKLQLRSCSLPKDFIT---------LLASAFHNNQNA	280
4	A.c.	ALTSIDWSNNLIKDAGVA---------ALAAAVASMGH	312
	C.e.	CLEYLDLSRNPIDDKKGFT--------ILSSVLPRLN	309
5	A.c.	GLTSISLKGGDATKKGTVALCTA-----FKKNVEMSR	344
	C.e.	QLKYVNFSECQLSDKSINLLCTG-----LYNGMTSCKSGGM	345
6	A.c.	TLTVLNLAGNRLDSDGTSA---------LAAFVSGPN	372
	C.e.	QLTELILSANPMK-DDISG---------IINLVSIST	372
7	A.c.	ALQTLNISGTAANLE------------MLLPAVMRGCT	398
	C.e.	SLRVLDFSDTGIHLD------------KLWNSLKFGGL	398
8	A.c.	ELEKFNISHNKVTAKTGP---------ELKKFLQSCG	426
	C.e.	QIEKLILNGCSLSKKSEGVQ-------TAKEYFSMAV	428
9	A.c.	RLSELHMRDTAVPVQVVRD--------VIKAIIGNNFIT	458
	C.e.	NLSHISFNNTSMPSEYLKA--------VLLGLASNQQLQ	460
10	A.c.	DF-QLDLAANKLGVLGAN---------MLAG-LAAEIT	484
	C.e.	PF-RLDLDAT-CEKGSAS---------VLDACIGGI	484
11	A.c.	TIKSLDLTDNDFGDEGMS---------IIADGLCHNS	512
	C.e.	RCETLSLRDNNLDGELQG---------VLQSLM-MVT	511
12	A.c.	SLRELHLGDN-WTRNKTKARSQAVDN--LIELI---SSEC	546
	C.e.	CLRRLDIGGSNMNQLKKNNKQVHVINKVLLDVVKLYSEEG	551
13	A.c.	PLHKLDLS-CKVADNQIKT--------DILPFIYSLATND	577
	C.e.	CLEELNLSDCRLGAYLS----------VLINTL--AATT	578
14	A.c.	TLKELDISGNAMGDKVGI---------ALGKAFQINR	605
	C.e.	TLKSLDISGNEMGNFGAR---------ILSKALQVNV	606
15	A.c.	TLSSLIWDRNGTRYAGFLG---------LKNGLERNN	633
	C.e.	SLRSVSIDNNHIGADGFVD---------LATSIKMNH	634
16	A.c.	VLVNMPVPVFDISD-LLKNEKPE----SAL-QVQQ	662
	C.e.	TLTHFPYPVHDAFDCMQRVERPR----TVA-ALSQ	664
Consensus		XLXXLXaXXnXaXXXXXXX±±±±±±±±aLaXaaXXXX±±±±

Preliminary alignment of Acan125 protein and C. elegans K07G5.1 gene product sequences by GCG BestFit were refined by inspection. The species are Acanthamoeba castellanii (A.c.) and Caenorhabditis elegans (C.e.). The C-terminal residue refers to the C terminus of the sequence shown, numbered from the initiator methionine. Letters in boldface indicate consensus residues. The consensus includes an L when >50% of the sequences contain leucine, an a when >80% of the sequences contain A, V, L, F, Y, or M, an n when >80% of the sequences contain N, C, or T, an X for an unspecified amino acid, and the symbol ± for an X that is variably present.