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. 1997 Apr 15;94(8):3691–3696. doi: 10.1073/pnas.94.8.3691

Table 2.

Effect of rpoD-RH596 in E. coli haploids

Activator Relative β-galactosidase activity
rpoD+ rpoD-RH596
None 15.0  ±  1.1 15.6  ±  1.6
cI wild type (100) 79.1  ±  4.3
cI-pc1 (Gly-43 to Arg) 4.7  ±  1.1 5.4  ±  1.2
cI-pc2 (Asp-38 to Asn) 15.5  ±  0.7 133  ±  10
cI-pc3 (Glu-34 to Lys) 0.6  ±  0.1 2.0  ±  0.5

Strains are derivatives of MS4274 and MS4276 carrying a PRM-lac8 ΔAB prophage plus either no second prophage or one of four Plac-cIA prophages. Four cultures of each strain were grown at 30°C in M9CAA (22), harvested in exponential phase, and assayed as described (23). Specific activities of the four control cultures (rpoD+ cI+) were averaged to yield the mean specific activity of the control (set at 100; corresponds to 131 Miller units). Specific activity of each of the other cultures was expressed as a percentage of the mean specific activity of the control. The four percentages for a particular genotype were used to calculate the mean and standard deviation given.