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Coexpression of GRIP1 in yeast increases the transactivation by RARs in response to at-RA ligand. Yeast transformants expressing different RAR receptor subtypes alone or with GRIP1 and containing β-gal reporter genes with either βRARE × 2 (Upper) or βRARE × 1 (Lower) enhancer elements were grown for 16 hr in the presence of no ligand (open bars) or 1 μM at-RA (shaded bars) and β-gal was assayed.
